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Arce Guerrero, Sandra Cruz Olivo, Edison Andrés González Morales, Shirley Moncada Quilindo, Carolina Valencia, Carlos Humberto 2020-03-25T19:57:26Z 2020-03-25T19:57:26Z 2015-07 0120-4319 http://red.uao.edu.co//handle/10614/12168 https://doi.org/10.11144/Javeriana.uo34-73.bftq Universidad Autónoma de Occidente Repositorio Institucional UAO Se busca desarrollar injertos óseos biocompatibles capaces de regenerar defectos óseos de tamaño crítico. Objetivo: Evaluar la biocompatibilidad in vivo del fosfato tricálcico con quitosano (FTQ) en piel, músculo y hueso. Metodología: Se asignaron 15 ratas Wistar a grupos A (piel), B (músculo), C, D y E (defectos óseos de tamaño crítico). Se implantó FTQ en cada tejido. Como control se colocaron esponjas de colágeno adyacente a los sitios evaluados. Las ratas de los grupos A y B se sacrificaron a los 20 días, mientras que las de los grupos C, D y E se sacrificaron a los 20, 40 y 80 días respectivamente. Para confirmar la biocompatibilidad del FTQ, se evaluó la respuesta inflamatoria en términos de porcentaje: ninguna (0 %), leve (˂30 %), moderada (30-50 %) y alta (˃50 %), después de 20, 40 y 80 días en el tejido óseo. Resultados: No se encontró ulceración ni supuración en piel, músculo o hueso. Después de 80 días, el FTQ se observaba incorporado a tejido fibrótico y oseointegrado al hueso nativo. Conclusión: El FTQ fue biocompatible in vivo en piel, músculo y hueso It is necessary to develop bone grafts capable to regenerate critical size bone defects. Objective: To evaluate the biocompatibility in vivo of tricalcium phosphate with chitosan (TPC) in skin, muscle, and bone. Methods: 15 Wistar rats were assigned to groups A (skin), B (muscle), C, D, and E (bone). TPC was placed in each tissue. In groups C-E, critical size bone defect was grafted with TPC and collagen sponge was placed adjacent to test sites as a control. Animals from groups A and B were sacrificed after 20 days, while groups C-E at days 45, 60, and 80. Inflammatory response was evaluated in all tissues. To assess biocompatibility, the percentage of cells was evaluated as none (0 %), low (˂ 30 %), moderate (30- 50 %), and high (˃50 %). Results: There were no signals of ulceration or suppuration in skin, muscle, and bone. After 80 days, TPC was incorporated into a fibrotic structure and osseointegrated to native bone Antecedentes: É necessário desenvolver enxertos ósseos capazes de regenerar defeitos ósseos de tamanho crítico. Objetivo: Avaliar a biocompatibilidade in vivo do fosfato tricalcico com quitosano (FTQ) na pele, músculo e osso. Métodos: 15 ratos Wistar foram seleccionados nos grupos A (pele), B (músculo), C, D e E (defeitos osseos de tamanho crítico). FTQ foi implantado em cada tecido. Esponjas de colágeno foram implantadas como controle ao lado do defeito ósseo. Os biomodelos dos grupos A e B foram sacrificados em 20 dias; e aos 20, 40, 80 dias nos grupos C, D e E respectivamente. Para conferir a biocompatibilidade do FTQ a resposta inflamatória foi avaliada em termos de porcentagem: Nenhuma (0%); leve (˂30%); moderada (30-50%); alta (˃50%) depois de 20, 40 e 80 dias no tecido ósseo. Resultados: Não houve ulceração nem supuração na pele, músculo e osso. Após de 80 dias, FTQ foi incorporado em um tecido fibroso e foi osseointegrado no osso nativo. Conclusão: O FTQ foi biocompatível in vivo na pele, músculo e osso application/pdf 8 páginas spa Pontificia Universidad Javeriana Universitas Odontológica. Volumen 34, número 73, (julio-diciembre 2015); páginas 109-116 116 73 109 34 Arce Guerrero, S.; Cruz Olivo, E. A.; González Morales, S.; Moncada Quilindo, C. ; Valencia, Carlos H. (2015). Biocompatibilidad del fosfato tricálcico con quitosano para uso en regeneración ósea. Universitas Odontológica. 34(73), 109-116. http://red.uao.edu.co//handle/10614/12168 Universitas Odontológica Mokbel N, Naaman N, Nohra J, Badawi N. Healing patterns of critical size bony defects in rats after grafting with bone substitutes soaked in recombinant human bone morphogenetic protein-2: histological and histometric evaluation. Br J Oral Maxillofac Surg. 2013; 51(6): 545-9 Nasr HF, Aichelmann-Reidy ME, Yukna RA. Bone and bone substitutes. Periodontology 2000. 1999; 19: 74-86. Rossi AC, Freire AR, Prado FB, Caria PHF. Bone substitutes used in dentistry. Int J Odontostomatol. 2014; 8(2): 289-98. Sogal A, Tofe AJ. Risk assessment of bovine spongiform encephalopathy transmission through bone graft material derived from bovine bone used for dental applications. J Periodontol. 1999; 70(9): 1053-63 Lee K, Weir MD, Lippens E, Mehta M, Wang P, Duda GN, et al. Bone regeneration via novel macroporous CPC scaffolds in critical-sized cranial defects in rats. 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Int J Oral Maxillofac Surg. 2015; 44(4): 513-7 Arce Guerrero S, Valencia Llano C, Garzón-Alvarado DA. Obtención de un biocompuesto constituido por fosfato tricálcico y quitosana para ser usado como sustituto óseo en un modelo animal. Rev Cub Inv Biomed. 2012; 31(3): 268-77 Ohura K, Bohner M, Hardouin P, Lemaitre J, Pasquier G, Flautre B. Resorption of, and bone formation from, new beta-tricalcium phosphate-monocalcium phosphate cements: An in vivo study. J Biomed Mater Res. 1996; 30(2): 193-200 Fernández T, Olave G, Valencia CH, Arce S, Quinn JMW, Thouas GA, et al. Effects of Calcium phosphate/chitosan composite on bone healing in rats: calcium phosphate induces osteon formation. Tissue Engineering Part A. 2014; 20(13-14): 1948-60 Klokkevold PR, Vandemark L, Kenney EB, Bernard GW. Osteogenesis enhanced by chitosan (poly-N-acetyl glucosaminoglycan) in vitro. J Periodontol. 1996; 67(11): 1170-5 Russel WMS, Burch RL. The principles of human experimental technique (1959): The global clearinghouse for information on alternatives to animal testing [Internet]. Baltimore, MD: Johns Hopkins University; 2014. Disponible en: http://altweb.jhsph.edu/pubs/books/humane_exp/het-toc Shukla SK, Mishra AK, Arotiba OA, Mamba BB. Chitosanbased nanomaterials: A state-of-the-art review. Int J Biol Macromol. 2013; 59: 46-58 Derechos Reservados - Pontificia Universidad Javeriana, 2015 https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) Biocompatibilidad del fosfato tricálcico con quitosano para uso en regeneración ósea Biocompatibility of tricalcium phosphate with chitosan for bone regeneration purposes Biocompatibilidade do fosfato tricalcico com quitosano para seu uso na regeneração óssea Artículo de revista http://purl.org/coar/resource_type/c_6501 Text info:eu-repo/semantics/article http://purl.org/redcol/resource_type/ARTREF info:eu-repo/semantics/publishedVersion Materiales biomédicos Tejido óseo Biomedical materials Bone tissue β-fosfato Biocompatibilidad Biomaterial Defecto óseo Hueso Quitosano Regeneración Tricálcico Alveolar bone loss β-tricalcium phosphate Biocompatibility Regeneration β-fosfato tricálcico http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/version/c_970fb48d4fbd8a85 Publication d07563aa-20d1-4a12-ba37-4d6839137e5a d07563aa-20d1-4a12-ba37-4d6839137e5a https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001112392open.access