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dc.contributor.authorPardo Flórez, Luz Marinaspa
dc.contributor.authorSalcedo Mendoza, Jairo Gspa
dc.contributor.authorGalán López, Jorge Enriquespa
dc.coverage.spatialUniversidad Autónoma de Occidente. Calle 25 115-85. Km 2 vía Cali-Jamundíspa
dc.date.accessioned2020-03-02T18:32:00Zspa
dc.date.available2020-03-02T18:32:00Zspa
dc.date.issued2012spa
dc.identifier.citationPardo Flórez, Luz Marina; Salcedo Mendoza, Jairo G.; Galán López, Jorge Enrique. Hidrólisis enzimática de residuos de la cosecha de caña de azúcar. En: Revista Colombiana de Biotecnología. Volumen 14, número 1, (2012); páginas 171-181spa
dc.identifier.issn1909-8758spa
dc.identifier.issn0123-3475spa
dc.identifier.urihttp://hdl.handle.net/10614/12016spa
dc.identifier.urihttps://revistas.unal.edu.co/index.php/biotecnologia/article/view/32797spa
dc.descriptionEn esta investigación, se hidrolizó un sustrato deslignificado proveniente de residuos de la cosecha caña de azúcar (hojas y cogollos) usando un preparado enzimático con 27.53 unidades de papel filtro (FPU), obtenido a partir de enzimas comerciales. La hidrólisis se llevó a cabo a un pH de 4.2 y una temperatura de 50 oC. Fueron analizados modelos de inhibición por sustrato, glucosa e inhibición total por producto. Los resultados mostraron que los modelos que mejor se ajustan a los datos experimentales, son los modelos de inhibición competitiva por glucosa, con una constante de Michaelis (Km) de 20.37 g/L, velocidad máxima (Vmax) 39 g/L h y una constante de inhibición (ki) de 0.442. En el caso que las relaciones enzima – Sustrato (E/S) sean mayores de 0.5, se puede aplicar el modelo cinético de Michaelis-Mentenspa
dc.description.abstractIn this research, a delignified substrate from crops residues sugar cane residues (leaves and top cane) was hydrolyzed using an enzyme preparation with 27.53 FPU. This enzyme was obtained from trade. Hydrolysis was carried out to pH of 4.2 and a temperature of 50 oC. Models of inhibition models substrate, glucose and total inhibition product was analyzed. The results showed that models that best fit the data experimental was the models competitive glucose inhibition (Km= 20.37, Vmax=39 and ki= 0.442). In the event that E/S is above 0.5, can applied kinetic models of Michaelis – Menteneng
dc.formatapplication/pdfspa
dc.format.extent171-181 páginasspa
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.relationRevista Colombiana de Biotecnología. Volumen 14, número 1, (2012); páginas 171-181spa
dc.rightsDerechos Reservados - Universidad Autónoma de Occidentespa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.sourceinstname:Universidad Autónoma de Occidentespa
dc.sourcereponame:Repositorio Institucional UAOspa
dc.subjectInhibiciónspa
dc.subjectModelos cinéticosspa
dc.subjectHojas y cogollosspa
dc.subjectCoctel de enzimasspa
dc.subjectInhibitioneng
dc.subjectKinetic modelseng
dc.subjectLeaves and tops caneeng
dc.subjectEnzyme cocktaileng
dc.titleHidrólisis enzimática de residuos de la cosecha de caña de azúcarspa
dc.title.alternativeHydrolysis Enzymatic of crop residues sugar caneeng
dc.typeArtículo de revistaspa
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