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dc.contributor.authorCastellanos, Angelicaspa
dc.contributor.authorGarcía, Lina Marcelaspa
dc.contributor.authorAstudillo, Myriamspa
dc.contributor.authorLópez Galán, Jorge Enriquespa
dc.contributor.authorFlórez Pardo, Luz Marinaspa
dc.coverage.spatialUniversidad Autónoma de Occidente. Calle 25 115-85. Km 2 vía Cali-Jamundíspa
dc.date.accessioned2019-11-20T19:30:20Zspa
dc.date.available2019-11-20T19:30:20Zspa
dc.date.issued2011spa
dc.identifier.citationCastellanos, Angélica; García, Lina Marcela; Astudillo, Myriam. Parámetros fisicoquímicos para la síntesis de ácido láctico o etanol de la bacteria (Corynebacterium glutamicum). En: Acta biológica Colombiana. Volumen 16, Número 2 (2011) páginas 15-32spa
dc.identifier.issn0120548X (impreso)spa
dc.identifier.issn19001649 (en línea)spa
dc.identifier.urihttp://hdl.handle.net/10614/11553spa
dc.identifier.urihttp://bdigital.unal.edu.co/19313/1/15280-90338-1-PB.pdfspa
dc.descriptionEl interés por obtener productos para la industria de biocombustibles a partir de desechos agrícolas, conduce a la búsqueda de nuevos sistemas biotecnológicos resistentes y costo-efectivos. Corynebacterium glutamicum, es un microorganismo usado para producir amino-ácidos que crece en gran variedad de sustratos y es resistente durante la fermentación, a variaciones de pH, temperatura, presión osmótica y acumulación de alcohol, características que lo hacen candidato a ser mejorado para la síntesis de ácido láctico y etanol. Aún se desconocen aspectos de su fisiología que aumenten su eficiencia en convertir azúcares (C5 y C6) en estos dos metabolitos. Por tanto, este trabajo buscó identificar los parámetros fisicoquímicos que tuvieron un mayor efecto sobre crecimiento bacteriano y síntesis de ácido láctico o etanol en un sistema por lotes. Para lograr este objetivo, ocho variables fueron evaluadas en un modelo estadístico producido en erlenmeyer; con los resultados obtenidos, se hallaron las mejores condiciones que fueron puestas a prueba en un cultivo en biorreactor. La temperatura, concentración de biotina y azúcar fueron las variables de mayor impacto (p< 0,05). Usando las mejores condiciones, 36 °C; 6,1 mg/L de biotina y 50 g/L de glucosa, se obtiene una μmax de 0,394 h-1, 16 g/L de ácido láctico a las 15 h del proceso con un rendimiento del 32%; observándose un mayor consumo de sustrato durante el crecimiento y poca dispo- nibilidad para la fermentación, sugiriendo una alimentación del cultivo al final de la fase exponencial que aumente los rendimientos de producción.spa
dc.description.abstractThe interest to obtain products for the bio-fuel industry from renewable resources has directed research to find resistant and costs-effective biotechnological systems. Corynebacterium glutamicum, is a microorganism used to produce amino acids, that grows in wide variety of substrates and its resistance during fermentation to pH, temperature, osmotic pressure variations and alcohol aggregate, renders this organism a suitable candidate to improve by genetic modifications lactic acid and ethanol synthesis. However, some aspects of its physiology remain unknown, such us increase lactic acid and ethanol production from C5 and C6 sugars. For this reason, the main aim in our work was to identify the most important variables with impact on culture and the best culture conditions to produce lactic acid or ethanol in batch culture. To achieve this objective, eight variables were tested in culture using a statistical model. The best culture conditions were obtained and tested in a bacth biorreactor system. Temperature, biotin and glucose concentration were the variables with most impact (p< 0.05) in culture. Using optimal conditions, 36 °C; 6.1 mg/L of biotin and 50 g/L of glucose; a μmax of 0.394 h-1, 16 g/L of lactic acid was obtained after 15 h of culture with an efficiency of 32%. High glucose consumption was observed during bacterial growth, which leads to low concentration of substrate for the production process; this suggests a culture feeding at the end of exponential growth phase, which can increase the production yieldeng
dc.formatapplication/pdfspa
dc.format.extentpáginas 15 - 31spa
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.relationActa biológica Colombiana. Volumen 16, Número 2 (2011) páginas 15-32spa
dc.rightsDerechos Reservados - Universidad Autónoma de Occidentespa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.sourceinstname:Universidad Autónoma de Occidentespa
dc.sourcereponame:Repositorio Institucional UAOspa
dc.subjectCorynebacterium glutamicumeng
dc.subjectFermentationeng
dc.subjectCorynebacterium glutamicumeng
dc.subjectBatch cultureeng
dc.subjectLactic acideng
dc.subjectFermentaciónspa
dc.subjectCultivo discontinuospa
dc.subjectAcido lácticospa
dc.titleParámetros fisicoquímicos para la síntesis de ácido láctico o etanol de la bacteria (Corynebacterium glutamicum)spa
dc.title.alternativePhysico-Chemical Parameter for Production of Lactic Acid or Ethanol of (Corynebacterium glutamicum) Bacteriaeng
dc.typeArtículo de revistaspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)spa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTREFspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
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